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GASTIER-FOSTER LABORATORY
Molecular Genetic Laboratory
The Molecular Genetics Laboratory is a CAP/CLIA certified laboratory with three specific functions: a clinical laboratory which performs:
- Genetic testing for inherited and somatic genetic disease
- A Children’s Oncology Group (COG) Acute Lymphoblastic Leukemia (ALL) Molecular Reference Laboratory
- A nucleic acid core facility for the COG and Gynecologic Oncology Group (GOG).
Clinical Laboratory
The clinical laboratory performs molecular genetic testing for inherited disease, as well as for applications to pediatric oncology. Of particular relevance to the Biopathology Center at Nationwide Children's Hospital, the laboratory performs RT-PCR analysis for the translocations associated with pediatric sarcomas (alveolar rhabdomyosarcoma, Ewing sarcoma, synovial sarcoma, desmoplastic small round cell tumor, and congenital fibrosarcoma/cellular mesoblastic nephroma).
COG ALL Molecular Reference Laboratory
The COG ALL Molecular Reference Laboratory is a section of the Biopathology Center within the Center for Childhood Cancer. The reference laboratory is responsible for the processing and banking of all legacy Children’s Cancer Group (CCG) ALL protocols, as well as half of the specimens for the new COG ALL clinical trials (Eastern Region). The samples for the legacy Pediatric Oncology Group (POG) ALL trials, as well as samples from the COG Western region are sent to the other Molecular reference laboratory at the University of New Mexico. The reference laboratories are responsible for the genetic analysis of pediatric ALL samples for treatment stratification and monitoring of disease state. Techniques include RT-PCR for the common pediatric ALL translocations, DNA ploidy analysis, and FISH. The reference laboratory also serves as a specimen repository for distribution of samples for additional pediatric ALL research. The ALL committee of the COG controls ALL sample distribution.
Nucleic Acid Core Facility
The nucleic acid facility is part of the Biopathology Center within the Center for Childhood Cancer. The laboratory is responsible for DNA and RNA extraction from tissue, bone marrow, and blood in support of COG and GOG protocols. The laboratory serves as a repository for distribution of nucleic acids to investigators for further research.
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| Education |
| 1999 |
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Board Certification |
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Clinical Molecular Genetics, Clinical Cytogenetics |
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Stanford University School of Medicine, Stanford, CA |
| 1996 |
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Ph.D. |
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Genetics |
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Harvard University, Boston, MA |
| Professional Experience |
| 2000- PRES |
Director, Molecular Genetics Laboratory, Biopathology Center, Center for Childhood Cancer, The Research Institute at Nationwide Children's Hospital, Columbus, OH |
| Selected Publications |
| Graef, I, Gastier, J.M., Francke, U., Crabtree, G.R. (2001). Evolutionary relationships among Rel domains
indicate functional diversification by recombination. Proc Natl Acad Sci USA. 98: 5740-5745. |
| Gastier, J.M., Berg, M.A., Vesterhus, P., Reiter, E., Francke, U. (2000). Diverse deletions in the
growth hormone receptor gene cause Growth Hormone Insensitivity Syndrome. Hum Mutation 16:323-333.
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| Ferguson, P.J., Blanton, S., Saulsbury, F.T., McDuffie, M.J., Lemahieu, V., Gastier, J.M., Francke,
U., Borowitz, S.M., Sutphen, J.L., Kelly, T.E. (2000). Manifestations and linkage analysis in X-linked
Autoimmunity-immunodeficiency Syndrome. Am. J. Med. Genet. 90: 390-397. |
| Lemahieu, V., Gastier, J.M., Francke, U. (1999). Novel mutations in the Wiskott-Aldrich Syndrome protein
gene and their effects on transcriptional, translational, and clinical phenotypes. Hum. Mutation. 14: 54-66. |
| Yandava, C.N., Gastier, J.M., Pulido, J.C., Brody, T., Sheffield, V., Murray, J., Buetow, K., Duyk,
G.M. (1997). Characterization of the Alu repeats that are associated with tri- and tetranucleotide repeat
microsatellites. Genome Research 7: 716-724. |
| Gastier, J.M., Brody, T., Pulido, J.C., Businga, T., Sunden, S., Hu, X., Maitra, S.,
Buetow, K.H., Murray, J.C., Sheffield, V.C., Boguski, M., Duyk, G.M., Hudson, T.J. (1996).
Development of a screening set for new (CAG/CTG)n dynamic mutations. Genomics 32: 75-85. |
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