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BESNER LABORATORY
Dr. Besner's research centers around a growth factor known as heparin-binding EGF-like growth factor (HB-EGF), first identified by Besner et al. in 1990. The central theme of Dr. Besner's research involves the effect of HB-EGF as an intestinal cytoprotective agent. Multiple lines of evidence, both in vitro and in vivo, have been obtained in Dr. Besner's laboratory to support the ability of HB-EGF to protect the intestines from injury. HB-EGF plays a vital role in postischemic regeneration due to its mitogenic, chemoattractant, antiapoptotic and anti-inflammatory effects. In vitro, HB-EGF is a potent mitogen and chemoattractant agent for intestinal epithelial cell lines. In addition, it protects intestinal epithelial cells from necrosis and apoptosis, in part by decreasing reactive oxygen and nitrogen radical formation. In vivo, we have used several rodent models of intestinal injury to demonstrate the intestinal cytoptotective effects of HB-EGF. We have shown that HB-EGF protects the intestine in the face of intestinal ischemia/reperfusion injury, hemorrhagic shock and resuscitation and experimental necrotizing enterocolitits. The translational component of Dr. Besner's research involves a disease process known as neonatal necrotizing enterocolitis (NEC), which results in intestinal necrosis in newborn babies, especially those born prematurely. The long range goal of Dr. Besner's work is the prophylactic and therapeutic treatment of high-risk neonates with HB-EGF, in order to prevent and treat this often devastating disease. In addition to funding of Dr. Besner's work from the National Institutes of Health, Dr. Besner is working with industry to bring HB-EGF to the bedside in human clinical trials, expected to begin in 2009.
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| Education |
| 1991 |
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Residency |
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Pediatric Surgery |
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Buffalo Children's Hospital, Buffalo, NY |
| 1989 |
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Fellowship |
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Surgical Research |
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Boston Children's Hospital Harvard Medical School, Boston, MA |
| 1987 |
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Residency |
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General Surgery |
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Brigham & Women’s Hospital, Boston, MA |
| 1982 |
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M.D. |
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Medicine |
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University of Cincinnati, Cincinnati, Ohio |
| Professional Experience |
| 2005- PRES |
Associate Program Director, Residency in Pediatric Surgery, Nationwide Children's Hospital and The Ohio State University College of Medicine, Columbus, Ohio |
| 2003- PRES |
Professor of Surgery and Pediatrics, The Ohio State University, Division of Pediatric Surgery at Nationwide Children's Hospital, Columbus, OH |
| 2001- PRES |
Member, Integrated Biomedical Sciences Graduate Program, The Ohio State University College of Medicine, Columbus, Ohio |
| 1994- Pres |
Member, Comprehensive Cancer Center, The Arthur James Cancer Hospital and Research Institute, Ohio State University, Columbus, OH |
| 1992- Pres |
Member, Molecular and Cellular Developmental Biology Program, Ohio State University, Columbus, OH |
| 2004- 2008 |
Full-time Member, Surgery, Anesthesia and Trauma Study Section, National Institutes of Health |
| 1997- 2003 |
Associate Professor of Surgery and Pediatrics, The Ohio State University, Division of Pediatric Surgery at Nationwide Children's Hospital, Columbus, OH |
| 1999- 2001 |
Co-Director, Medical Scientist Training Program, Ohio State University, Columbus, OH |
| 1991- 1997 |
Assistant Professor of Surgery and Pediatrics, Ohio State University, Division of Pediatric Surgery at Columbus Children's Hospital, Columbus, OH |
| 1991- 1991 |
Pediatric Surgeon, Children's Hospital, Buffalo, NY |
| 1989- 1989 |
Surgical Research, Children's Hospital, Boston, MA |
| 1987- 1989 |
Research Fellow, Department of Surgery, Children's Hospital and Harvard Medical School, Boston, MA
Mentors: Dr. M. Judah Folkman and Dr. Michael Klagsbrun
Project: Identification of a novel growth factor (heparin-binding EGF-like growth factor (HB-EGF)) from cultured human macrophages. |
| 1987- 1987 |
Surgeon, Brigham & Women's Hospital, Boston, MA |
| 1977- 1978 |
Undergraduate Research Fellowship, Cornell University, Division of Nutritional Sciences, Ithaca, NY
Mentor: Dr. V. Utermohlen
Project: The effect of exogenously added fatty acids on cell mediated immunity. |
BESNER LABORATORY STAFF
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Principal Investigator:
Gail E. Besner, M.D.
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| Selected Publications |
| Radulescu A, Yu X, Orvets N, Chen Y, Zhang H-Y, Besner G: Deletion of the Heparin-binding EGF-like Growth Factor Gene Increases Susceptibility to Necrotizing Enterocolitis. J Pediatr Surg 45:729-734, 2010. |
| Chen C-L, Mehta V, Wu D, Otabor I, El-Assal O, Feng J, Radulescu A, Zhang H-Y, Chen Y, Besner G: Intestinal Phenotype in Mice Overexpressing a Heparin-Binding EGF-like Growth Factor Transgene in Enterocytes. Growth Factors 28:82-97, 2010. |
| Christensen RD, Wiedmeier SE, Baer VL, Henry E, Gerday E, Lambert DK, Burnett J, Besner GE: Antecedents of Bell Stage III Necrotizing Enterocolitis. J Perinatology. 30:54-57, 2010. |
| Otabor I, Chen C-L, Huang G, Zhang H-Y, Velten M, Besner G: Heparin-Binding EGF-Like Growth Factor Protects the Lungs from Remote Injury After Intestinal Ischemia/Reperfusion. J Surg Res (in press). |
| Askegard-Giesmann J, Besner G, Fabia R, Caniano D, Preston T, Kenney B: Extracorporeal Membrane Oxygenation (ECMO) as a Life-Saving Modality in the Treatment of Pediatric Patients with Burns and Respiratory Failure. J Pediatr Surg (in press). |
| Zhang H-Y, Radulescu A, Chen Y, Besner G: Heparin-Binding EGF-like Growth Factor (HB-EGF) Improves Intestinal Microcirculation after Hemorrhagic Shock and Resuscitation in Mice. J Surg Res 2010 (EPub ahead of print). |
| Zhang H-Y, Radulescu R, Chen C-L, Olson J, Darbyshire A, Besner G: Mice Overexpressing the Gene for Heparin-Binding EGF-Like Growth Factor (HB-EGF) Have Increased Resistance to Hemorrhagic Shock and Resuscitation. Surgery (submitted). |
| Yu X, Radulescu A, Chen C-L, Besner G: Heparin-Binding EGF-like Growth Factor Protects Pericytes from Injury. Microvascular Research (submitted). |
| Besner G: Pediatric Burn Management. Fundamentals of Pediatric Surgery, Peter Mattei (ed) (in press). |
| Christensen R, Gordon P, Besner G: Can We Cut the Incidence of Necrotizing Enterocolitis in Half – Today? Fetal and Pediatric Pathology 29:1-14, 2010. |
| Zhou Y, Brigstock, D, Besner G: HB-EGF is a Potent Dilator of Terminal Mesenteric Arterioles. Microvascular Research 78:78-85, 2009. |
| Yu X, Radulescu A, Zorko N, Besner G: Heparin-Binding EGF-like Growth Factor Increases Intestinal Microvascular Blood Flow in Necrotizing Enterocolitis. Gastroenterology 137:221-230, 2009. Special Honor: Journal Cover FigureSpecial Honor: Video Abstract hosted by YouTube |
Otabor I, Besner G: Burns: Surgical Perspective. In Scoll Plantz (ed): Pediatrics: E-Medicine, 2009.
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Otabor I, Balant J, Besner G: Myectomy-Myotomy for Long Segment Hirschprung’s Disease in a Patient with Haddad Syndrome. J Pediatr Surg 44:620-622, 2009. |
Radulescu A, Zorko N, Yu X, Besner G: Preclinical Neonatal Rat Studies of HB-EGF in Protection of the Intestines from Necrotizing Enterocolitis. Pediatr Res 65:437-442, 2009 |
Otabor I, Erdman S, Abdessalam S, Besner G: Gastric Outlet Obstruction due to Adenocarcinoma in a Patient with Ataxia-Telangiectasia Syndrome: Case Report and Review of the Literature. World Journal of Surgical Oncology 7:29, 2009. |
| Zhang, H-Y, Radulescu A, Besner G: Heparin-Binding EGF-Like Growth Factor is Essential for Preservation of Gut Barrier Function after Hemorrhagic Shock and Resuscitation in Mice. Surgery 146: 334-339, 2009. |
| El-Assal O, Paddock H, Marquez A, Besner G: HB-EGF Gene Disruption is Associated with Impaired Angiogenesis, Delayed Intestinal Restitution, and Poor Survival after Intestinal Ischemia in Mice. J Pediatr Surg 43:1182-1190, 2008. |
Mehta V, Zhou Y, Radulescu A, Besner G: HB-EGF Stimulates eNOS Expression and Promotes eNOS Dependent Angiogenesis. Growth Factors 26:301-315, 2008. |
Arora A, Caniano D, Hammond S, Besner G: Inversion Appendectomy as a Lead Point for Intussusception. Pediatr Surg Int 24:1261-1264, 2008. |
El-Assal O, Paddock H, Marquez A, Besner G: HB-EGF Gene Disruption is Associated with Impaired Angiogenesis, Delayed Intestinal Restitution, and Poor Survival after Intestinal Ischemia in Mice. J Pediatr Surg 43:1182-1190, 2008 |
Shiels W, Kenney B, Caniano D, Besner G: Definitive Percutaneous Treatment of Lymphatic Malformations of the Trunk and Extremities. J Pediatr Surg 43:136-139, 2008. |
Mehta V, Besner G: HB-EGF Promotes Angiogenesis in Endothelial Cells via PI3-kinase and MAPK Signaling Pathways. Growth Factors 25:253-263, 2007. |
| Feng J and Besner G: HB-EGF Promotes Enterocyte Proliferation and Migration in Neonatal Rats with NEC. J Pediatr Surg 42:214-220, 2007. |
| Rocourt D, Mehta V, Besner G: Heparin-Binding EGF-Like Growth Factor (HB-EGF) Decreases Pro-Inflammatory Cytokine Expression after Intestinal Ischemia/Reperfusion Injury. J Surg Res 139:269-273, 2007. |
| Rocourt D, Mehta V, Besner G: Heparin-Bidning EGF0Like Growth Factor (HB-EGF) Decreases Neutrophil-Endothelial Cell Interactions. J Surg Res 141:262-266, 2007 |
| El-Assal O, Radulescu A, Besner G: HB-EGF Preserves Mesenteric Microcirculatory Blood Flow and Protects against Intestinal Injury in Rats Subjected to Hemorrhagic Shock and Resuscitation. Surgery 142:234-242, 2007. |
| Mehta V, Besner G: HB-EGF Promotes Angiogenesis in Endothelial Cells via P13-kinase and MAPK Signaling Pathways. Growth Factors 25:253-263, 2007. |
Mehta V, Besner G: HB-EGF Promotes Angiogenesis in Endothelial Cells via PI3-kinase and MAPK Signaling Pathways. Growth Factors 25:253-263, 2007. |
El-Assal O, Radulescu A, Besner G: HB-EGF Preserves Mesenteric Microcirculatory Blood Flow and Protects against Intestinal Injury in Rats Subjected to Hemorrhagic Shock and Resuscitation. Surgery 142:234-242, 2007. |
Rocourt D, Mehta V, Besner G: Heparin-Binding EGF-like Growth Factor (HB-EGF) Decreases Neutrophil-Endothelial Cell Interactions. J Surg Res 141:262-266, 2007. |
Lambert DK, Christensen RD, Henry E, Besner GE, Baer VL, Wiedmeier SE, Stoddard RA, Miner C, Burnett J: Necrotizing Enterocolitis in Term Neonates: Data From a Multihospital Healthcare System. J Perinatology 27: 437-443, 2007. |
Rocourt D, Mehta V, Besner G: Heparin-Binding EGF-Like Growth Factor (HB-EGF) Decreases Pro-Inflammatory Cytokine Expression after Intestinal Ischemia/Reperfusion Injury. J Surg Res 139:269-273, 2007. |
Paddock H, Fabia R, Giles S, Hayes J, Lowell W, Besner G: A Silver Impregnated Antimicrobial Dressing Reduces Hospital Length of Stay for Pediatric Burn Patients. J Burn Care Research 28:409-11, 2007. |
| Feng J, El-Assal O, Besner G: Heparin-Binding EGF-Like Growth Factor Decreases the Incidence of Necrotizing Enterocolitis in Neonatal Rats. J Pediatr Surg 41:144-149, 2006. |
| Feng J, El-Assal O, Besner G: Heparin-Binding EGF-Like Growth Factor (HB-EGF) Reduces Intestinal Apoptosis in Neonatal Rats with NEcrotizing Enterocolitis. J Pediatr Surg 41:742-747, 2006. |
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